Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers

Authors

DOI:

https://doi.org/10.21498/2518-1017.4(33).2016.88678

Keywords:

varietal purity, typicality, hybridity, sterility, DNA markers, SSR markers, quality control of seed lots, barley

Abstract

Purpose. To develop a conceptually new method for determination of varietal purity (typicality), hybridity, steri­lity of seed lots.

Methods of molecular biology (genomic DNA extraction, PCR with SSR markers application, capillary electrophoresis), genetic, statistical, mathematical analysis.

Results. New method for determining the varietal qualities of seed lot was developed that consists of the following steps: simultaneous DNA extraction from a representative sample of aggregated seeds; PCR and further analysis of the amplification products by determination of the qualitative and quantitative composition of SSR-markers’ alleles; calculation of values of varietal seed lot quality using experimentally derived allele ratios.

Conclusions. The developed method for determining varietal qualities of seed lots allows to reduce significantly the consumption of materials, time and labor during the analysis. Consistent qualification and quantification of alleles in the total sample of a seed lot is a conceptually new approach to establish varietal purity (typicality), hybridity, sterility.

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Author Biographies

Ж. В. Вдовиченко, National University of Life and Environmental Sciences of Ukraine

Zhanna Vdovychenko

В. Г. Спиридонов, National University of Life and Environmental Sciences of Ukraine

Vladislav Spyrydonov

С. В. Хомутовська, National University of Life and Environmental Sciences of Ukraine

Stanislava Khomutovska

М. Ф. Парій, National University of Life and Environmental Sciences of Ukraine

Myroslav Parii

References

Leonova, I. N. (2013). Molecular markers: implementation in crop plant breeding for identification, introgression, and gene pyramiding. Vavilovskij Žurnal Genetiki i Selekcii [Vavilov Journal of Genetics and Breeding], 17(2), 314–325. [in Russian]

Sivolap, Yu. M. (2013). Molecular markers and plant breeding. Cytol Genet., 47(3), 188–195. doi: 10.3103/S0095452713030080

Newbury, H. J. (Ed.). (2003). Plant Molecular Breeding. Birmingham, UK: Blackwell Publ., CRC Press.

Rafalski, A. (2002). Applications of single nucleotide polymorphisms in crop genetics. Curr Opin Plant Biol., 5(2), 94–100. doi: 10.1016/S1369-5266(02)00240-6

Becker, J., & Heun, M. (1995). Barley microsatellites: allele variation and mapping. Plant Mol. Biol., 27(4), 835–845. doi: 10.1007/BF00020238

Edwards, D., & Batley, J. (2010). Plant genome sequencing: applications for crop improvement. Plant Biotechnol J., 8(1), 2–9. doi: 10.1111/j.1467-7652.2009.00459.x

Gut, I. G. (2001). Automation in genotyping of single nucleotide polymorphisms. Hum Mutat., 17(6), 475–492. doi: 10.1002/humu.1131

Sozinov, A. A. (1985). Polimorfizm belkov i ego znachenie v genetike i selektsii [Polymorfism of proteins and its importance in genetics and breeding]. Moscow: Nauka. [in Russian] Anisimova, I. N. (1987). Identification of varieties, lines and hybrids for the composition of helianthine polypeptides. Sbornik nauchnych trudov po prikladnoy botanike, genetike i selektsii [Proceedings on Applied Botany, Genetics and Bree­ding], 114, 114–126. [in Russian]

Syvolap, Yu. M., & Kozhukhova, N. E. (2005). DNA techniques in the registration and protection of plant varieties rights. Sortovivčennâ ohor. prav sorti roslin [Plant Varieties Studying and Protection], 1, 66–74. doi: 10.21498/2518-1017.1.2005.66849. [in Ukrainian]

Rybalka, O. I., Chervonis, M. V., & Lytvynenko, M. A. (2008). Genetic heterogeneity of wheat varieties developed in Odesa for allelic composition of GLI/GLU loci. Visnyk ahrarnoi nauky [Вulletin of Agricultural Science], 2, 54–59. [in Ukrainian]

Balvinska, M. S., Volkova, N. E., Kolesnyk, O. O., Solodenko, A. Ye., & Chebotar, S. V. (2015). Dyferentsiatsiia, identyfikatsiia, vyznachennia typovosti ta hibrydnosti silskohospodarskykh kultur za DNK-profiliuvanniam [Differentiation, identification, determination of typicality and hybridity of cultivated crops for DNA profiling]. Odesa: Astroprynt. [in Ukrainian]

Akinina, G. E., Terenyak, Yu. N., Sharypina, Ya. Yu., & Popov V. N. (2016). Genetic purity of seeds – actual question of modern genetics and plant breeding. Fakt. eksp. evol. org. [Factors in experimental evolution of organisms], 18, 56–60. [in Russian]

McPherson, M. J., & Moller, S. G. (2006). PCR (pp. 209–232). (2nd ed.). New York: Taylor & Francis Group.

Hoogendoorn, B., Norton, N., Kirov, G., Williams, N, Hamshere, M. L., Spurlock, G., … O’Donovan, M. C. (2000). Cheap, accurate and rapid allele frequency estimation of single nucleotide polymorphisms by primer extension and DHPLC in DNA pools. Hum Genet., 107(5), 488–493.

Parii, M. F., Vdovychenko, Zh. V., & Spyrydonov, V. H. (2011). Method for determining varietal purity and typicalness of lots of seed of farm crops using dna-markers. Ukraine patent for invention, МПК7 А 01Н 1/04. – No. u 2007 11049. [in Ukrainian].

Ramsay, L., Macaulaya, M., degli Ivanissevich, S., MacLean, K., Cardle, L., Fuller, J., … Waugh, R. (2000). A simple sequence repeat-based linkage map of barley. Genetics, 156(4), 1997–2005.

Keb-Llanes, M., Gonźales, G., Chi-Manzanero, B., & Infante, D. (2002). A rapid and simple method for small scale DNA extraction in Agavaceae and other tropical plants. Plant Mol. Biol. Rep., 20(3), 299a–299e.

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Published

2016-12-28

How to Cite

Вдовиченко, Ж. В., Спиридонов, В. Г., Хомутовська, С. В., & Парій, М. Ф. (2016). Method for determination of varietal purity (typicality), hybridity, sterility of seed lots based on the establishment of the quantitative ratio of alleles of DNA markers. Plant Varieties Studying and Protection, (4(33), 56–62. https://doi.org/10.21498/2518-1017.4(33).2016.88678

Issue

No. 4(33) (2016)

Section

BIOTECHNOLOGY AND BIOSAFETY

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Copyright (c) 2016 Ж. В. Вдовиченко, В. Г. Спиридонов, С. В. Хомутовська, М. Ф. Парій

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